| 释义 |
polymerase Biochem.|ˈpɒlɪməreɪz, pəˈlɪməreɪz|
[f. polymer + -ase.]
Any enzyme which catalyses the formation of a polymer, esp. a polynucleotide.
1958I. R. Lehman et al. in Jrnl. Biol. Chem. CCXXXIII. 163/2 In order to facilitate reference in this report, the enzyme responsible for deoxyribonucleotide incorporation is designated as ‘polymerase’. 1964New Scientist 23 Jan. 211/1 DNA has been bio-synthesised from its four basic building blocks put together with the enzyme polymerase and some natural DNA to act as a template. 1973B. J. Williams Evolution & Human Origins vi. 89/1 RNA polymerase is active when the cell is carrying on its normal metabolic functions.
▸ polymerase chain reaction n. Molecular Biol. a technique for producing many copies of a chosen sequence of genomic DNA using repeated cycles of denaturation, annealing with primer, and replication with DNA polymerase; abbreviated PCR.
1985R. K. Saiki et al. in Science 20 Dec. 1350/3 The β-globin segment was amplified by the *polymerase chain reaction (PCR) procedure of Mullis and Faloona. 1996P. H. Duesberg Inventing AIDS Virus vi. 180 The very ability of retroviruses to survive as dormant genes by attaching themselves to human chromosomes has been exploited for the most sensitive HIV assay yet—the Polymerase Chain Reaction. 2000New Scientist 11 Nov. 89/1 (advt.) Applications are invited for a Technician to work on a project to detect micrometastases in primary breast cancer patients using a combination of immunocytochemistry and quantitative polymerase chain reaction. |