| 释义 |
Definition of sonicate in English: sonicateverb ˈsɒnɪkeɪtˈsänikāt [with object]Biochemistry Subject (a biological sample) to ultrasonic vibration so as to fragment the cells, macromolecules, and membranes. cells were sonicated in an ice bath Example sentencesExamples - Samples were sonicated for 5 sec four times to shear genomic DNA and reduce viscosity.
- The samples were sonicated for 12 sec and cell number was analyzed using a Coulter counter.
- The sample was sonicated to clarity in a sonication bath (Ultrawave).
- After dialysis the residue was sonicated and ready for use.
- The samples were sonicated for 30 min, followed by centrifugation and overnight incubation at 65°C as described above.
noun ˈsɒnɪkeɪtˈsänikāt Biochemistry A biological sample which has been sonicated. Example sentencesExamples - Reactions were initiated by the addition of crude cell sonicates to prewarmed solutions.
- Finally, the sonicate in the third cuvette was additionally treated with DNase for another 1 h at 25 deg C, then Cyan 40 was added and the emission spectrum was recorded.
- The sonicate was cleared by centrifugation at 12,000 g for 30 minutes at 4°C.
- The sonicate was centrifuged for 10 min at 16,000 RCF and the resulting supernatant collected.
- The sonicate was cleared by microcentrifugation for 5 min at 4° and mixed with the anti-HA.11 antibody coupled to Sepharose beads (prewashed three times with lysis buffer).
Derivatives noun Biochemistry Cells were disrupted by sonication, and soluble recombinant proteins were purified on the TALON metal-affinity resin (Clontech). Example sentencesExamples - Lipid hydration was coupled with six cycles of freezing, thawing, and sonication, except sonication was omitted in the last cycle.
- Chromatin was sheared by sonication, and DNA associated with either CLF1 or ORC1 was separately co-immunoprecipitated using an anti-HA antibody.
- To test whether the brief low-power sonication changes the solubility limit of cholesterol in a bilayer, we performed a control experiment with 16: 0,18: 1PC.
- The following day, cells were harvested by centrifugation, resuspended in buffer (20 mM Tris-HCl, pH 8.0; 100 mM NaCl), and disrupted by sonication.
Origin 1950s: from sonic + -ate2. Definition of sonicate in US English: sonicateverbˈsänikāt [with object]Biochemistry Subject (a biological sample) to ultrasonic vibration so as to fragment the cells, macromolecules, and membranes. cells were sonicated in an ice bath Example sentencesExamples - The sample was sonicated to clarity in a sonication bath (Ultrawave).
- The samples were sonicated for 30 min, followed by centrifugation and overnight incubation at 65°C as described above.
- After dialysis the residue was sonicated and ready for use.
- The samples were sonicated for 12 sec and cell number was analyzed using a Coulter counter.
- Samples were sonicated for 5 sec four times to shear genomic DNA and reduce viscosity.
nounˈsänikāt Biochemistry A biological sample which has been sonicated. Example sentencesExamples - The sonicate was cleared by centrifugation at 12,000 g for 30 minutes at 4°C.
- The sonicate was cleared by microcentrifugation for 5 min at 4° and mixed with the anti-HA.11 antibody coupled to Sepharose beads (prewashed three times with lysis buffer).
- The sonicate was centrifuged for 10 min at 16,000 RCF and the resulting supernatant collected.
- Finally, the sonicate in the third cuvette was additionally treated with DNase for another 1 h at 25 deg C, then Cyan 40 was added and the emission spectrum was recorded.
- Reactions were initiated by the addition of crude cell sonicates to prewarmed solutions.
Origin 1950s: from sonic + -ate. |