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单词 Northern blot
释义

Definition of Northern blot in English:

Northern blot

noun
Biology
  • An adaptation of the Southern blot procedure used to detect specific sequences of RNA by hybridization with complementary DNA.

    Example sentencesExamples
    • We and others have previously shown that CHO cells do not express detectable amounts of endogenous RyRs when assayed by Western blots, Northern blots, or ryanodine binding.
    • Southern and Northern blots contained nucleic acids from 10 million P. carinii per lane.
    • In this case a major band was detected on Northern blots using either a P-derived probe or a cDNA probe, which corresponds to misexpression of the major unspliced RNA.
    • Standard techniques were used for DNA cloning, restriction enzyme digestion, Southern and Northern blot analysis, and DNA sequencing.
    • Equal RNA loading was monitored on the agarose gel under UV light or by hybridizing the Northern blot with a radiolabelled 28 S ribosomal RNA cDNA fragment isolated from Nicotiana tabacum cv.
 
 

Definition of Northern blot in US English:

Northern blot

noun
Biology
  • An adaptation of the Southern blot procedure used to detect specific sequences of RNA by hybridization with complementary DNA.

    Example sentencesExamples
    • Equal RNA loading was monitored on the agarose gel under UV light or by hybridizing the Northern blot with a radiolabelled 28 S ribosomal RNA cDNA fragment isolated from Nicotiana tabacum cv.
    • Standard techniques were used for DNA cloning, restriction enzyme digestion, Southern and Northern blot analysis, and DNA sequencing.
    • In this case a major band was detected on Northern blots using either a P-derived probe or a cDNA probe, which corresponds to misexpression of the major unspliced RNA.
    • Southern and Northern blots contained nucleic acids from 10 million P. carinii per lane.
    • We and others have previously shown that CHO cells do not express detectable amounts of endogenous RyRs when assayed by Western blots, Northern blots, or ryanodine binding.
 
 
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更新时间:2024/12/23 8:09:31