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单词 autoradiograph
释义

Definition of autoradiograph in English:

autoradiograph

noun ɔːtəʊˈreɪdɪəɡrɑːfˌôdōˈrādēəɡraf
  • A photograph of an object produced by radiation from radioactive material in the object.

    Example sentencesExamples
    • We assessed band sharing on polyacrylamide gel autoradiographs.
    • The chromatogram produced can then be used to give an autoradiograph so the compounds that the radioactive material has been incorporated into can be readily identified.
    • The autoradiographs were scanned with a densitometer, and the signals were quantified.
    • Hybridization can be visualized by measuring color development on the blot when enzyme labels are used or by developing the radioactive blots onto autoradiographs.
    • We scored autoradiographs by identifying novel and shared bands.
    • Fig 3 and Fig 4 each show scanned images of autoradiographs from a single gel and a single exposure.
verb ɔːtəʊˈreɪdɪəɡrɑːfˌôdōˈrādēəɡraf
[with object]
  • Make an autoradiograph of.

    the gel was dried and autoradiographed
    Example sentencesExamples
    • Blots were dried and then autoradiographed for 5-15 days at - 80&deg.
    • Following chromatography, the plate was dried and autoradiographed.
    • The membranes were then autoradiographed using a phosphoimaging system.
    • Samples were electrophoresed on a 7% SDS-polyacrylamide gel, and autoradiographed.
    • The radiolabelled products were separated in a 6% denaturing polyacrylamide gel and autoradiographed.
    • The labelled reaction products resulting from elongation of the primers were separated on polyacrylamide gels which were then autoradiographed.
    • Alternatively, the gel was autoradiographed at - 80°C.
    • The washed membrane was autoradiographed overnight.
    • Radioactivity in blots was detected upon exposure to autoradiograph film.
    • After the pre-treatment of the gel, it is autoradiographed and the retarded band is cut from the gel and placed in an eppendorf tube.

Derivatives

  • autoradiographic

  • adjective ˌɔːtəreɪdɪəˈɡrafɪk
    • Membranes were exposed to autoradiographic film for 4 hours.
      Example sentencesExamples
      • Hybridization was visualized using autoradiographic exposure to film.
      • Signals from hybridized filters were recorded on autoradiographic films.
      • For quantitative analysis, both the autoradiographic standards and the autoradiographs were initially scanned.
      • In the case of radioactive probes, caution is to be exercised in interpreting the autoradiographic signal at the edge of the section and at the inner surface of blood vessels or airways (because of the ‘edge effect’).
  • autoradiography

  • noun ˌɔːtəreɪdɪˈɒɡrəfi
    • The gels were dried and subjected to autoradiography and analyzed by computer-assisted densitometry.
      Example sentencesExamples
      • Radioactivity on the membrane was detected by autoradiography.
      • After washing, the membrane was subjected to autoradiography for 48 hours at -70 degrees C.
      • Hybridized transcripts were visualized by autoradiography.
      • If your main interest is clinical an explanation of intracellular recordings and autoradiography will be welcome, and presumably scientists will have a similar view about the clinical sections.
 
 

Definition of autoradiograph in US English:

autoradiograph

nounˌôdōˈrādēəɡraf
  • A photograph of an object produced by radiation from radioactive material in the object and revealing the distribution or location of labeled material in the object.

    Example sentencesExamples
    • We assessed band sharing on polyacrylamide gel autoradiographs.
    • The chromatogram produced can then be used to give an autoradiograph so the compounds that the radioactive material has been incorporated into can be readily identified.
    • The autoradiographs were scanned with a densitometer, and the signals were quantified.
    • Fig 3 and Fig 4 each show scanned images of autoradiographs from a single gel and a single exposure.
    • Hybridization can be visualized by measuring color development on the blot when enzyme labels are used or by developing the radioactive blots onto autoradiographs.
    • We scored autoradiographs by identifying novel and shared bands.
verbˌôdōˈrādēəɡraf
[with object]
  • Make an autoradiograph of.

    the gel was dried and autoradiographed
    Example sentencesExamples
    • After the pre-treatment of the gel, it is autoradiographed and the retarded band is cut from the gel and placed in an eppendorf tube.
    • Radioactivity in blots was detected upon exposure to autoradiograph film.
    • The washed membrane was autoradiographed overnight.
    • Alternatively, the gel was autoradiographed at - 80°C.
    • The labelled reaction products resulting from elongation of the primers were separated on polyacrylamide gels which were then autoradiographed.
    • The membranes were then autoradiographed using a phosphoimaging system.
    • Following chromatography, the plate was dried and autoradiographed.
    • The radiolabelled products were separated in a 6% denaturing polyacrylamide gel and autoradiographed.
    • Blots were dried and then autoradiographed for 5-15 days at - 80&deg.
    • Samples were electrophoresed on a 7% SDS-polyacrylamide gel, and autoradiographed.
 
 
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更新时间:2024/11/13 4:17:50