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单词 deproteinize
释义

Definition of deproteinize in English:

deproteinize

(British deproteinise)
verb diːˈprəʊtiːnʌɪzdēˈprōtēəˌnīz
[with object]
  • Remove the protein from (a substance), usually as a stage in chemical purification.

    Example sentencesExamples
    • DNA (proteinase K-extracted, stored frozen) was RNase treated and then deproteinized by a standard phenol-chloroform extraction.
    • Serum samples were deproteinized with 10 per cent perchloric acid, vortexed for 60 sec for mixing and centrifugation was done at 4000 rpm for 10 min.
    • For the ELD experiments, calf thymus DNA was deproteinized with sodium dodecyl sulfate.
    • For the determination of glutathione, the sample obtained as described for enzyme assays was deproteinized immediately using 30% HClO 4.
    • One ml serum separated by centrifugation, was deproteinised by the addition of 100 l perchlorate.

Derivatives

  • deproteinization

  • noundiːprəʊtiːnʌɪˈzɛɪʃ(ə)n
    • The Cd concentration was measured according to a modified deproteinization method using a flameless atomic absorption spectrophotometer.
      Example sentencesExamples
      • Immunoprecipitation, deproteinization, and PCR were performed as described.
      • The analysis of the electrophoretic mobility of DNA was performed after repeated pronase treatment and deproteinization in chloroform-isoamyl alcohol.
      • Genomic DNA extractions were carried out from 0.2 g of adult flies, as described by Piñol et al., except that a phenol deproteinization step was added before deproteinization with chloroform.
      • Plasma NOx was similarly assayed after deproteinization with cold ethanol.
 
 

Definition of deproteinize in US English:

deproteinize

(British deproteinise)
verbdēˈprōtēəˌnīz
[with object]
  • Remove the protein from (a substance), usually as a stage in chemical purification.

    Example sentencesExamples
    • For the ELD experiments, calf thymus DNA was deproteinized with sodium dodecyl sulfate.
    • Serum samples were deproteinized with 10 per cent perchloric acid, vortexed for 60 sec for mixing and centrifugation was done at 4000 rpm for 10 min.
    • One ml serum separated by centrifugation, was deproteinised by the addition of 100 l perchlorate.
    • DNA (proteinase K-extracted, stored frozen) was RNase treated and then deproteinized by a standard phenol-chloroform extraction.
    • For the determination of glutathione, the sample obtained as described for enzyme assays was deproteinized immediately using 30% HClO 4.
 
 
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更新时间:2024/9/20 19:38:44