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单词 electroporation
释义

Definition of electroporation in English:

electroporation

noun ɪˌlɛktrə(ʊ)pəˈreɪʃ(ə)niˌlektrōpəˈrāSHən
mass nounBiology
  • The action or process of introducing DNA or chromosomes into bacteria or other cells using a pulse of electricity to open the pores in the cell membranes briefly.

    Example sentencesExamples
    • In molecular biology, the process of electroporation is often used for the transformation of bacteria, yeast, and plant protoplasts.
    • DNA was introduced into DH10ß cells via electroporation with a Gene Pulser II (Bio-Rad).
    • Plasmids pJF138 and pJF141 were introduced into cells by electroporation.
    • For electroporation, cells are also grown to mid-log phase but are then washed extensively with water to eliminate all salts.

Derivatives

  • electroporate

  • verb ɪˌlɛktrə(ʊ)pəˈreɪtəˌlɛktroʊpəˈreɪt
    [with object]Biology
    • Subject to electroporation.

      the tumour was electroporated using a circular array electrode
      Example sentencesExamples
      • Plasmid DNA was electroporated into 60 l of electrocompetent Escherichia coli C and incubated in a shaker/incubator for 30 min at 37°C for the positive selection of mutants.
      • This plasmid was electroporated into Agrobacterium tumefaciens strain GV3101, which was used to transform pex5-1 mutant plants by the floral dip method.
      • Early observations that poly tails can enhance expression of reporter mRNAs electroporated into plant protoplasts were interpreted as evidence for the role of poly tails in translation in plants.
 
 

Definition of electroporation in US English:

electroporation

nouniˌlektrōpəˈrāSHən
Biology
  • The action or process of introducing DNA or chromosomes into bacteria or other cells using a pulse of electricity to briefly open the pores in the cell membranes.

    Example sentencesExamples
    • DNA was introduced into DH10ß cells via electroporation with a Gene Pulser II (Bio-Rad).
    • Plasmids pJF138 and pJF141 were introduced into cells by electroporation.
    • In molecular biology, the process of electroporation is often used for the transformation of bacteria, yeast, and plant protoplasts.
    • For electroporation, cells are also grown to mid-log phase but are then washed extensively with water to eliminate all salts.
 
 
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更新时间:2024/12/23 9:19:31