centrifugation
cen·tri·fuge
C0203300 (sĕn′trə-fyo͞oj′)| Noun | 1. | centrifugation - the process of separating substances of different densities by the use of a centrifuge |
| 单词 | centrifugation | |||
| 释义 | centrifugationcen·tri·fugeC0203300 (sĕn′trə-fyo͞oj′)
CentrifugationCentrifugationA mechanical method of separating immiscible liquids or solids from liquids by the application of centrifugal force. This force can be very great, and separations which proceed slowly by gravity can be speeded up enormously in centrifugal equipment. Centrifugal force is generated inside stationary equipment by introducing a high-velocity fluid stream tangentially into a cylindrical-conical chamber, forming a vortex of considerable intensity. Cyclone separators based on this principle remove liquid drops or solid particles from gases, down to 1 or 2 μm in diameter. Smaller units, called liquid cyclones, separate solid particles from liquids. The high velocity required at the inlet of a liquid cyclone is obtained with standard pumps. Much higher centrifugal forces than in stationary equipment are generated in rotating equipment (mechanically driven bowls or baskets, usually of metal, turning inside a stationary casing). Rotating a cylinder at high speed induces a considerable tensile stress in the cylinder wall. This limits the centrifugal force which can be generated in a unit of a given size and material of construction. Very high forces, therefore, can be developed only in very small centrifuges. There are two major types of centrifuges: sedimenters and filters. A sedimenting centrifuge contains a solid-wall cylinder or cone rotating about a horizontal or vertical axis. An annular layer of liquid, of fixed thickness, is held against the wall by centrifugal force; because this force is so large compared with that of gravity, the liquid surface is essentially parallel with the axis of rotation regardless of the orientation of the unit. Heavy phases “sink” outwardly from the center, and less dense phases “rise” inwardly. Heavy solid particles collect on the wall and must be periodically or continuously removed. A filtering centrifuge operates on the same principle as the spinner in a household washing machine. The basket wall is perforated and lined with a filter medium such as a cloth or a fine screen; liquid passes through the wall, impelled by centrifugal force, leaving behind a cake of solids on the filter medium. The filtration rate increases with the centrifugal force and with the permeability of the solid cake. Some compressible solids do not filter well in a centrifuge because the particles deform under centrifugal force and the permeability of the cake is greatly reduced. The amount of liquid adhering to the solids after they have been spun also depends on the centrifugal force applied; in general, it is substantially less than in the cake from other types of filtration devices. See Mechanical separation techniques Centrifugationthe separation of inhomogeneous systems, such as suspensions, slurries, and emulsions, by means of centrifugal force. Centrifugation may be carried out by settling or by filtration. A machine in which centrifugation is performed is called a centrifuge. A centrifuge that operates on the basis of settling has an unperforated rotor; the process of centrifuging with such a machine is known as centrifugal clarification. A centrifuge whose operation is based on filtration has a perforated rotor with a filter medium covering the inner surface. Centrifugal clarification is used to separate the clarified liquid phase from a dilute suspension or to precipitate the solid phase from a concentrated suspension. Centrifugal filtration is most often employed to separate the solid phase from a suspension or slurry containing a relatively small amount of liquid. Ultracentrifugation is used to investigate highly disperse systems and high-molecular-weight compounds, such as proteins. REFERENCESSee references under .centrifugationcentrifugation[sen-trif″u-ga´shun]cen·trif·u·ga·tion(sen-trif'yū-gā'shŭn),centrifugationLab technology The process of separating fractions of systems in a centrifuge. See Axial centrifugation, Density gradient centrifugation, Ultracentrifugation.cen·trif·u·ga·tion(sen-trif'ŭ-gā'shŭn)centrifugationa process in which particles in suspension are subjected to centrifugal forces by being spun in a CENTRIFUGE, so forming a sediment. Subsequent removal of the SUPERNATANT fluid from the sediment and subjecting it to higher speeds of centrifugation can enable particles of different sizes to be separated out and collected. Eventually the supernatant fluid will contain only soluble materials. In some types of centrifugation, the sample is layered on to a column of liquid (such as sucrose) of increasing concentration down the column, and centrifuged until bands are formed. In this way the molecules are separated by their different rates of sedimentation. see DIFFERENTIAL CENTRIFUGATION, ULTRACENTRIFUGE.centrifugation
Words related to centrifugation
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