fluorescence polarization immunoassay


fluorescence polarization immunoassay

, fluorescent polarization immunoassay Abbreviation: FPIA
A means of identifying and quantifying the amount of an antigen in a specimen in which a fluorescently labeled antibody is mixed with a sample thought to contain the antigen is exposed to polarized light. Bound fluorescently labeled antibody reacts to polarized light in a characteristic fashion not demonstrated by unbound antibody, allowing for identification and measurement of the ligand. See also: immunoassay