| Acronym | Definition |
|---|
| DFO➣Department of Fisheries and Oceans (Canada) |
| DFO➣Designated Federal Official |
| DFO➣Disaster Field Office (US FEMA) |
| DFO➣Derived Format Object |
| DFO➣Dispatcher Failover |
| DFO➣Data Flow Object |
| DFO➣Data Flow Operator |
| DFO➣Darkfall Online (online gaming) |
| DFO➣Deferoxamine |
| DFO➣Divisional Forest Officer |
| DFO➣Dairy Farmers of Ontario (Canada) |
| DFO➣Director of Financial Operations (various organizations) |
| DFO➣Director of Flight Operations (airline rank) |
| DFO➣Distillate Fuel Oil |
| DFO➣Direct Factory Outlet (Australian retail center) |
| DFO➣District Forest Office |
| DFO➣Dartmouth Flood Observatory |
| DFO➣Drilling Fluid Operator (offshore oil & gas) |
| DFO➣1,8-Diazafluoren-9-One (forensic chemical) |
| DFO➣Director of Field Operations |
| DFO➣Dolphins Fanatics Online (football website) |
| DFO➣Done Fell Out |
| DFO➣Dislodged Foreign Object (gaming; World of Warcraft) |
| DFO➣Deutscher Falkenorden (German Falcon Order) |
| DFO➣Des Filles en Ovalie (French: Girls in Rugby) |
| DFO➣Diesel Fuel Oil |
| DFO➣Digital Fuel Optimizer (RevTech motorcycle fuel control system) |
| DFO➣Dell Factory Outlet |
| DFO➣Donor Funded Operation (World Bank) |
| DFO➣Date File Opened (US DHS) |
| DFO➣Done Fell Over |
| DFO➣Dining Facility Operation |
| DFO➣Distance from Origin |
| DFO➣Dungeon Fighter Online (video game) |
| DFO➣Divisional Finance Officer |
| DFO➣Documentation for Operations |
| DFO➣Duty Field Officer (UK) |
| DFO➣Dumb Freakin' Operator (polite form) |
| DFO➣Diploma in Front Office (various educational institutions) |