单词 | chromosome walking |
释义 | chromosome walkingchromosome walking[′krō·mə‚sōm ‚wȯk·iŋ]chromosome walkingchro·mo·some walk·ingchromosome walkingA procedure for determining the nucleotide sequence of segments of DNA that are located on either side of a region of fully characterised (i.e., “sequenced”) DNA. Chromosome walking requires the dividing (“walking out”) of each segment of DNA into overlapping short (15–20,000) bases from a point of a known sequence; this enables one to sequence DNA far removed from a marker gene.chro·mo·some walk·ing(krō'mŏ-sōm wawk'ing)chromosome walkinga technique for mapping chromosomes that involves the sequential isolation of clones carrying overlapping NUCLEOTIDE SEQUENCES. This makes it possible to ‘walk’ along the chromosome from one gene to the next, to reach a desired LOCUS. Chromosome walking is used in genome sequencing projects, see CLONE CONTIG SEQUENCING METHOD. |
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