Western blotting

[′wes·tərn ‚bläd·iŋ] (cell and molecular biology) A protein detection technique in which proteins are separated by one- or two-dimensional gel electrophoresis, transferred (blotted) to a nitrocellulose sheet, and then treated with radioactive antibodies (or antibodies coupled to a fluorescent dye or an easily detectable enzyme) that are specific to the protein of interest.

Western blotting

A method of detecting very small quantities of a protein of interest in a cell or body fluid. The sample is spread by ELECTROPHORESIS on a block of polyacrylamide gel. The separated proteins are then transferred, by blotting, to a thin plastic sheet to make them more accessible for reaction with an antibody specific for the protein of interest. The antibody-antigen complex can then be detected by rinsing the sheet with a second, radioactive, antibody that recognises the first. The sheet will now produce a dark band on X-ray film. See also SOUTHERN BLOTTING.

Western transfer

Western blotting

a method for transferring proteins, that have been separated by GEL ELECTROPHORESIS, from the gel to a membrane support. The method is named by analogy with SOUTHERN TRANSFER.

Western blotWestern blot analysis, , Western blotting (wes'tĕrn blot, blot'ing) Synonym(s): Western blot analysis.

单词	western blotting
释义	DictionarySeeWestern Blot Western blotting Western blotting [′wes·tərn ‚bläd·iŋ] (cell and molecular biology) A protein detection technique in which proteins are separated by one- or two-dimensional gel electrophoresis, transferred (blotted) to a nitrocellulose sheet, and then treated with radioactive antibodies (or antibodies coupled to a fluorescent dye or an easily detectable enzyme) that are specific to the protein of interest. Western blotting Western blotting A method of detecting very small quantities of a protein of interest in a cell or body fluid. The sample is spread by ELECTROPHORESIS on a block of polyacrylamide gel. The separated proteins are then transferred, by blotting, to a thin plastic sheet to make them more accessible for reaction with an antibody specific for the protein of interest. The antibody-antigen complex can then be detected by rinsing the sheet with a second, radioactive, antibody that recognises the first. The sheet will now produce a dark band on X-ray film. See also SOUTHERN BLOTTING. Western transfer or Western blotting a method for transferring proteins, that have been separated by GEL ELECTROPHORESIS, from the gel to a membrane support. The method is named by analogy with SOUTHERN TRANSFER. Western blotWestern blot analysis, , Western blotting (wes'tĕrn blot, blot'ing) Synonym(s): Western blot analysis. AcronymsSeewine bar
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