reverse transcriptase polymerase chain reaction


reverse transcriptase polymerase chain reaction (RT-PCR),

a process for specific mRNA amplification wherein reverse transcriptase added to the in vitro reaction uses mRNA as a template to produce one cDNA, which is then amplified by the usual PCR.

re·verse trans·crip·tase pol·y·mer·ase chain re·ac·tion

(RT-PCR) (rē-vĕrs' tran-skrip'tās pol-im'ĕr-ās chān rē-ak'shŭn) A process for specific mRNA amplification wherein reverse transcriptase added to the in vitro reaction uses mRNA as a template to produce one cDNA, which is then amplified by the usual PCR.