| 释义 |
reverse genetics
reverse genetics[ri¦vərs jə′ned·iks] (cell and molecular biology) An experimental method in which information from cloned deoxyribonucleic acid (DNA) or protein sequences is used to find or to produce mutations that help identify the function of a gene or protein (in contrast to classical genetics in which a known function or trait is traced back to a particular gene. reverse genetics
reverse geneticsa conceptual approach allowing the study of gene function; it can be summarized as "from gene to phenotype" as opposed to forward genetics, which can be summarized as "from phenotype to gene." It uses structural changes in the gene by deletion through gene knockout or disruption by site-directed mutagenesis, and then investigating the effects of such alterations on the phenotype of the cell or organism. Synonym(s): positional cloning |