Southern blotting

[′sə·th·ərn ‚bläd·iŋ] (cell and molecular biology) A technique for the detection of specific sequences among deoxyribonucleic acid (DNA) fragments whereby the fragments are separated by gel electrophoresis and then blotted onto a sheet of nitrocellulose for detection with radioactively labeled nucleic acid probes. It was first described by E. M. Southern in 1975.

Southern blotting

A method developed by EM Southern for detecting and manipulating specific DNA sequences previously separated by gel electrophoresis using a radioactively- or chemically-labeled DNA probe with sequence similarity

Southern blotting

A method of identifying a fragment of DNA containing a specific sequence of bases. A mixture of fragments, produced by cutting DNA with restriction enzymes, is separated on a gel block by ELECTROPHORESIS. The fragments are denatured to single strand DNA and transferred, by blotting, to a nitrocellulose sheet. The position of the desired fragment can then be shown by hybridizing it with a DNA probe labelled with radioactive phosphorous that will cause a black line on an X-ray film. The method was named after its developer Edward M. Southern. A similar technique for RNA analysis has been humorously called ‘Northern blotting’ and the play on words has been extended to include ‘WESTERN BLOTTING’.

Southern transfer

Southern blotting

a method of transferring BANDS of DNA, separated by GEL ELECTROPHORESIS, to a membrane support. The technique is named after E. Southern, who devised it. Compare NORTHERN TRANSFER.

单词	southern blotting
释义	DictionarySeeSouthern blot Southern blotting Southern blotting [′sə·th·ərn ‚bläd·iŋ] (cell and molecular biology) A technique for the detection of specific sequences among deoxyribonucleic acid (DNA) fragments whereby the fragments are separated by gel electrophoresis and then blotted onto a sheet of nitrocellulose for detection with radioactively labeled nucleic acid probes. It was first described by E. M. Southern in 1975. Southern blotting A method developed by EM Southern for detecting and manipulating specific DNA sequences previously separated by gel electrophoresis using a radioactively- or chemically-labeled DNA probe with sequence similarity Southern blotting A method of identifying a fragment of DNA containing a specific sequence of bases. A mixture of fragments, produced by cutting DNA with restriction enzymes, is separated on a gel block by ELECTROPHORESIS. The fragments are denatured to single strand DNA and transferred, by blotting, to a nitrocellulose sheet. The position of the desired fragment can then be shown by hybridizing it with a DNA probe labelled with radioactive phosphorous that will cause a black line on an X-ray film. The method was named after its developer Edward M. Southern. A similar technique for RNA analysis has been humorously called ‘Northern blotting’ and the play on words has been extended to include ‘WESTERN BLOTTING’. Southern transfer or Southern blotting a method of transferring BANDS of DNA, separated by GEL ELECTROPHORESIS, to a membrane support. The technique is named after E. Southern, who devised it. Compare NORTHERN TRANSFER.
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