释义 |
Phase-Contrast Microscopy
Phase-Contrast Microscopy a research technique in microscopy that uses special equipment to produce high-contrast images of colorless, transparent objects with different structure densities, such as living microoganisms and tissue cultures. phase-contrast microscopy
phase-contrast microscopy a microscopy technique for viewing colourless transparent objects by exploiting the interference between diffracted and undiffracted beams of light to produce an increase in contrast. When such an object is viewed through a light microscope the resulting image has a uniform brightness though the light emerging from different parts of the object has different phase relations which are due to variations in the refractive index. Phase-contrast microscopy uses these phase differences to give an image that would be produced had these differences been in the amplitude of the light. |