sucrose hemolysis test


sucrose

 [soo´krōs] a disaccharide" >disaccharide obtained from sugar cane, sugar beet, or other sources; used as a food and sweetening agent.sucrose hemolysis test a test for hemoglobinuria" >paroxysmal nocturnal hemoglobinuria; the patient's whole blood is mixed with isotonic sucrose solution, which promotes binding of complement to red blood cells, then incubated and examined for hemolysis; greater than 10 per cent hemolysis indicates paroxysmal nocturnal hemoglobinuria.

su·crose he·mol·y·sis test

isotonic sucrose promotes binding of complement to red blood cells; in paroxysmal nocturnal hemoglobinuria a proportion of the cells is sensitive to complement-mediated lysis, and hemolysis ensues.

sucrose hemolysis test

A screening test for paroxysmal nocturnal haemoglobinuria (PNH), which is based on binding of complement to red cell membranes. In the test, red cells from a patient are incubated in isotonic saline mixed with sucrose in barbital. Lysis of > 10% of tested red cells is typical of PNH.