radioimmunosorbent test


radioimmunosorbent test

 (RIST) [ra″de-o-im″u-no-sor´bent] a radioiummunoassay technique for measuring serum IgE concenration, using radiolabeled IgE and anti–human IgE bound to an insoluble matrix. The patient's IgE competes with the radiolabeled IgE attached to the matrix, so that the decrease in radiolabel remaining attached to the matrix can be used to determine the total concentration of serum IgE in the patient.

ra·di·o·im·mun·o·sor·bent test (RIST),

a competition test, performed in vitro, used to measure IgE specific for a particular antigen. Known amounts of radiolabeled IgE compete with the patient's unlabeled IgE to bind to a surface coated with anti-IgE. The reduction in radiolabeled IgE due to the presence of IgE in the patient's serum can be determined by comparison with known IgE standards; thus, the amount of the patient's total serum IgE can be determined.

ra·di·o·im·mun·o·sor·bent test

(RIST) (rā'dē-ō-im'yū-nō-sōr'bĕnt test) A competition assessment, performed in vitro, used to measure IgE specific for a particular antigen. Known amounts of radiolabeled IgE compete with the patient's unlabeled IgE to bind to a surface coated with anti-IgE. The reduction in radiolabeled IgE due to the presence of IgE in the patient's serum can be determined by comparison to known IgE standards; thus, the amount of the patient's total serum IgE can be determined.