| 释义 |
site-directed mutagenesis
site-·di·rec·ted mu·ta·gen·e·sisthe controlled alterations of selected regions of a DNA molecule.site-directed mutagenesis An experimental technique in which a mutation is inserted in DNA at a particular site to determine the site’s function, if any. In site-directed mutagenesis, nucleotides are altered, resulting in substitution of amino acids in a protein of known function, which serves to identify the DNA sequence’s role (e.g., as a receptor or ion channel). |