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单词 northern blotting
释义

Northern blotting


Northern blotting

[¦nȯrth·ern ′bläd·iŋ] (genetics) Method of ribonucleic acid (RNA) detection and identification in which the intact RNA is separated by size via gel electrophoresis, transferred (blotted) onto nitrocellulose or nylon paper, and then hybridized with labeled DNA probes.

Northern blotting


A technique used to detect a specific mRNA sequence, which consists of electrophoresing RNA fragments, transferring those fragments to a membrane, then adding a labelled nucleic acid probe to the milieu

Northern blotting

Molecular biology A technique used to detect the presence of a specific mRNA sequence. See Blotting, Hybridization, Probe, RNA, Southern blotting.

Northern transfer

or

Northern blotting

a method for transferring BANDS of RNA, separated by GEL ELECTROPHORESIS, from the electrophoresis gel to a membrane support. The method is named by analogy with SOUTHERN TRANSFER.
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