A significant advance in clone library construction for physical mapping and large-scale sequencing was the development of the bacterial artificial chromosome (BAC) vector in 1992. BAC vectors stably propagate DNA with insert size from 80 to 300 kb. Lee Rowen et al., Science
2
blood alcohol concentration
At a BAC of .10 percent, sensory and motor functions become noticeably impaired. Speech becomes slurred, and people have difficulty coordinating arm and leg movements. Rita L. Atkinson et al., Introduction to Psychology, 9th edition