The analysis revealed the capability of mangiferin to reversibly inhibit elastase and collagenase activity.
Renata Ochocka, Anna Hering, Justyna Stefanowicz–Hajduk, Krzysztof Cal, Helena Barańska 2017, 'The effect of mangiferin on skin: Penetration, permeation and inhibition of ECM enzymes',PLOS ONE10.1371/journal.pone.0181542. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Adherent neutrophils were isolated from the lung vasculature by collagenase digestion and sequential filtering.
Laskin Jeffrey D, Gordon Marion K, Zhou Peihong, Connor Agnieszka J, Sunil VasanthiR, Laskin Debra L 2002, 'Activation of adherent vascular neutrophils in the lung during acute endotoxemia',Respiratory Researchhttp://dx.doi.org/10.1186/rr171. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Scaffolds showed more resistance to collagenase digestion.
Akhshabi S, Biazar E, Singh V, Keshel SH, Geetha N 2018, 'The effect of the carbodiimide cross-linker on the structural and biocompatibilityproperties of collagen-chondroitin sulfate electrospun mat', International Journal of Nanomedicinehttps://www.dovepress.com/the-effect-of-the-carbodiimide-cross-linker-on-the-structural-and-bioc-peer-reviewed-article-IJN. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
The inhibitory activities against collagenase, elastase, hyaluronidase, and tyrosinasewere employed for anti-aging.
Pham Thi Be Tu, Shinkichi Tawata 2015, 'Anti-Oxidant, Anti-Aging, and Anti-Melanogenic Properties of the Essential Oils fromTwo Varieties of Alpinia zerumbet', Moleculeshttp://www.mdpi.com/1420-3049/20/9/16723. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
In the next group, the collagen membrane was soaked in both collagenase and growth factors.
B. Gurer, S. Cabuk, O. Karakus, N. Yilmaz, C. Yilmaz 2018, 'In vivo cartilage tissue engineering', Journal of Orthopaedic Surgery and Researchhttp://link.springer.com/article/10.1186/s13018-018-0823-0. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
In the other group, the collagen membrane was soaked in collagenase prior to usage.
B. Gurer, S. Cabuk, O. Karakus, N. Yilmaz, C. Yilmaz 2018, 'In vivo cartilage tissue engineering', Journal of Orthopaedic Surgery and Researchhttp://link.springer.com/article/10.1186/s13018-018-0823-0. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
These homogenized lipoaspirates were then mixed with collagenase and incubated.
Jaewoo Pak, Jung Hun Lee, Kwang Seung Park, Byeong Chul Jeong, Sang Hee Lee 2016, 'Regeneration of Cartilage in Human Knee Osteoarthritis with Autologous Adipose Tissue-DerivedStem Cells and Autologous Extracellular Matrix', BioResearch Open Accesshttp://online.liebertpub.com/doi/full/10.1089/biores.2016.0024. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Collagenase-inhibitor interaction was studied theoretically by computational calculations.
Sergio Roberto de Andrade Leite 2009, 'Inhibitors of human collagenase, MMP1', Eclética Químicahttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-46702009000400008. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
After 1 to 4 days of incubation, collagenase in the supernatant was assayed with collagen.
Fazwishni Siregar, S.M. M.S. Akbar 2007, 'Jatropha curcas latex inhibits the release of collagenase by gingival fibroblast',Medical Journal of Indonesiahttp://mji.ui.ac.id/journal/index.php/mji/article/view/282. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
The lipoaspirate was digested with collagenase and washed with saline three times.
Vickers ER, Karsten E, Flood J, Lilischkis R 2014, 'A preliminary report on stem cell therapy for neuropathic pain in humans', Journal of Pain Researchhttp://www.dovepress.com/a-preliminary-report-on-stem-cell-therapy-for-neuropathic-pain-in-huma-a16748. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)