Urea and sodium chloride showed no sporicidal activity.
Enrique Acosta-Gío, Aurelio Herrero-Farías, Víctor Hugo Mata-Portuguez 2001, 'El cloruro de benzalconio: inaceptable para esterilizar o desinfectar instrumentalmédico o dental Benzalkonium chloride is unacceptable to sterilize or disinfect medicalor dental instruments', Salud Pública de Méxicohttp://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0036-36342001000600008. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Surface tests are preferable for evaluating disinfectants with sporicidal effects on hard surfaces.
Marie Christine Uwamahoro, Richard Massicotte, Yves Hurtubise, François Gagné-Bourque,Akier Assanta Mafu, L'Hocine Yahia 2018, 'Evaluating the Sporicidal Activity of Disinfectants against Clostridium difficileand Bacillus amyloliquefaciens Spores by Using the Improved Methods Based on ASTME2197-11', Frontiers in Public Healthhttp://journal.frontiersin.org/article/10.3389/fpubh.2018.00018/full. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Appropriate sporicidal disinfectants are needed to prevent development of clusters and outbreaks.
N. Kenters, E. G. W. Huijskens, S. C. J. de Wit, I. G. J. M. Sanders, J. van Rosmalen,E. J. Kuijper, A. Voss 2017, 'Effectiveness of various cleaning and disinfectant products on Clostridium difficilespores of PCR ribotypes 010, 014 and 027', Antimicrobial Resistance and Infection Controlhttp://link.springer.com/article/10.1186/s13756-017-0210-3. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Nowadays, the sporicidal properties of plants have been considered as alternatives to chemical sporicidal agents.
Vajihe Karbasizade, Arezoo Dabiri 2014, 'Evaluation of the Sporicidal Activity of Ethanol Extract of Arctium lappa Root againstBacillus cereus', Majallah-i taḥqīqāt-i ̒ulūm-i pizishkī-i Zāhidānhttp://www.zjrms.ir/browse.php?a_id=2894&sid=1&slc_lang=en. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Following the selection of the optimum method of antimicrobial activity neutralization, two disinfectant concentrations were examined for their sporicidal activity.
Mostafa Essam Eissa, Mohab Abd El Naby, Mohamed Mohamed Beshir 2014, 'Bacterial vs. fungal spore resistance to peroxygen biocide on inanimate surfaces',Bulletin of Faculty of Pharmacy, Cairo Universityhttp://www.sciencedirect.com/science/article/pii/S1110093114000362. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
The sporicidal activity of acidified ethanol was enhanced by increasing ionic strength and mild elevations in temperature.
Michelle M Nerandzic, Venkata C K Sunkesula, Thriveen Sankar C, Peter Setlow, CurtisJ Donskey 2015, 'Unlocking the Sporicidal Potential of Ethanol: Induced Sporicidal Activity of Ethanolagainst Clostridium difficile and Bacillus Spores under Altered Physical and ChemicalConditions.', PLoS ONEhttp://europepmc.org/articles/PMC4503543?pdf=render. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)