an enzyme that is capable of detaching the terminal nucleotide from a nucleic acid chain
Compare endonuclease
Examples of 'exonuclease' in a sentence
exonuclease
Sequence analysis also revealed extensive exonuclease activity, providing additional diversity.
Jenni Liljavirta, Mikael Niku, Tiina Pessa-Morikawa, Anna Ekman, Antti Iivanainen 2014, 'Expansion of the preimmune antibody repertoire by junctional diversity in Bos taurus.',PLoS ONEhttp://europepmc.org/articles/PMC4057420?pdf=render. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
The expected ~10nt peak was seen, and showed strong elevation in strains lacking exonuclease activity.
Clémentine Delan-Forino, Claudia Schneider, David Tollervey 2017, 'RNA substrate length as an indicator of exosome interactions in vivo [version 2; referees:3 approved]', Wellcome Open Researchhttps://wellcomeopenresearch.org/articles/2-34/v2. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
We find that this occurs in both their wild-type (exo+) and exonuclease deficient (exo-) forms.
Melissa G. eCastillo-Lizardo, Ghislaine eHenneke, Enrique eViguera 2014, 'Replication slippage of the thermophilic DNA polymerases B and D from the EuryarchaeotaPyrococcus abyssi', Frontiers in Microbiologyhttp://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00403/full. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Ian F. Connerton, Steven P.T. Hooton 2015, 'Campylobacter jejuni acquire new host-derived CRISPR spacers when in association withbacteriophages harbouring a CRISPR-like Cas4 protein', Frontiers in Microbiologyhttp://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00744/full. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Here we consider the practical details of exonuclease attachment to the protein alpha hemolysin.
Richard M. A. Manara, Susana Tomasio, Syma Khalid 2015, 'The Nucleotide Capture Region of Alpha Hemolysin: Insights into Nanopore Design forDNA Sequencing from Molecular Dynamics Simulations', Nanomaterialshttp://www.mdpi.com/2079-4991/5/1/144. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
The newly discovered exonuclease lacks similarity to any other proteins with known function,including hitherto reported 3′–5′ exonucleases.
Kazuo Tori, Sonoko Ishino, Shinichi Kiyonari, Saki Tahara, Yoshizumi Ishino 2013, 'A Novel Single-Strand Specific 3′–5′ Exonuclease Found in the Hyperthermophilic Archaeon,Pyrococcus furiosus', PLoS ONE10.1371/journal.pone.0058497. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Many mutations map to the polymerase and exonuclease domains of the enzyme and produce a broad clinical spectrum.
Chardot Christophe, Kern Ilse, Jackson Christopher B, Hahn Dagmar, Schaller André,Belli Dominique C, Gallati Sabina, Nuoffer Jean-Marc 2011, 'Molecular and biochemical characterisation of a novel mutation in POLG associatedwith Alpers syndrome', BMC Neurologyhttp://www.biomedcentral.com/1471-2377/11/4. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Exonuclease digested the single-strand aptamer and released the thrombin, which was then detected in the next recycle.
Gao L, Li Q, Deng Z, Brady B, Xia N, Zhou Y, Shi H 2017, 'Highly sensitive protein detection via covalently linked aptamer to MoS2 and exonuclease-assistedamplification strategy', International Journal of Nanomedicinehttps://www.dovepress.com/highly-sensitive-protein-detection-via-covalently-linked-aptamer-to-mo-peer-reviewed-article-IJN. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)