an enzyme that is responsible for scission of a nucleic acid chain, the action of which is not confined to the terminal nucleotide
Compare exonuclease
endonuclease in American English
(ˌendouˈnuːkliˌeis, -ˌeiz, -ˈnjuː-)
noun
Biochemistry
any of a group of enzymes that degrade DNA or RNA molecules by breaking linkages within the polynucleotide chains
Word origin
[1960–65; endo- + nuclease]This word is first recorded in the period 1960–65. Other words that entered Englishat around the same time include: bicycle kick, buyback, deinstitutionalize, pop art, tag questionendo- is a combining form meaning “within,” used in the formation of compound words. Otherwords that use the affix endo- include: endobiotic, endocrine, endocytosis, endodermis, endogenous
Examples of 'endonuclease' in a sentence
endonuclease
Plasmid diversity was assessed by endonuclease digestion.
Jane W. Marsh, Mary G. Krauland, Jemma S. Nelson, Jessica L. Schlackman, Anthony M.Brooks, A. William Pasculle, Kathleen A. Shutt, Yohei Doi, Ashley M. Querry, CarleneA. Muto, Lee H. Harrison 2015, 'Genomic Epidemiology of an Endoscope-Associated Outbreak of Klebsiella pneumoniaeCarbapenemase (KPC)-Producing K. pneumoniae', PLOS ONE10.1371/journal.pone.0144310. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Gene duplication also happened to an endonuclease-like gene.
Daniel M. P. Ardisson-Araújo, Ana Maria Rodrigues da Silva, Fernando L. Melo, EthianeRozo dos Santos, Daniel R. Sosa-Gómez, Bergmann M. Ribeiro 2018, 'A Novel Betabaculovirus Isolated from the Monocot Pest Mocis latipes (Lepidoptera:Noctuidae) and the Evolution of Multiple-Copy Genes', Viruseshttp://www.mdpi.com/1999-4915/10/3/134. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
No topoisomerase or endonuclease activity was detected.
Mikhailov Victor S, Rohrmann George F 2002, 'Binding of the baculovirus very late expression factor 1 (VLF-1) to different DNAstructures', BMC Molecular Biologyhttp://www.biomedcentral.com/1471-2199/3/14. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
These ribozymes generally spread through endonuclease-mediated intron homing.
Simon Dawn M, Reeb Valérie, Bhattacharya Debashish, Lutzoni François 2005, 'Phylogenetic analyses suggest reverse splicing spread of group I introns in fungalribosomal DNA', BMC Evolutionary Biologyhttp://www.biomedcentral.com/1471-2148/5/68. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Gene polymorphism was evaluated by the polymerase chain reaction followed by restriction endonuclease digestion.
Messias Antônio Araújo, Bruno Soares Menezes, Clauber Lourenço, Elisângela Rosa Cordeiro,Renata Ríspoli Gatti, Luiz Ricardo Goulart 2005, 'O gene do angiotensinogênio (M235T) e o infarto agudo do miocárdio The angiotensinogengene (M235T) and the acute myocardial infarction', Revista da Associação Médica Brasileirahttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302005000300018. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Finally, they were ligated to the restriction-endonuclease digested vectors.
Ye-jiang Lou, Jie Jin 2017, 'Insert restriction enzyme cutting-free cloning strategy for expression plasmid construction',Biotechnology & Biotechnological Equipmenthttp://dx.doi.org/10.1080/13102818.2017.1351310. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
The proteins were inserted at the site of the lost endonuclease.
Davis Trisha N, Arms Luther, Ramsden Richard, Muller Eric GD 2011, 'An intein with genetically selectable markers provides a new approach to internallylabel proteins with GFP', BMC Biotechnologyhttp://www.biomedcentral.com/1472-6750/11/71. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Viral isolates were typed by restriction endonuclease analysis.
Serdar TUNCER, Mehmet CEYHAN, Kadriye YURDAKÖK, Güler KANRA, Şemsettin USTAÇELEBİ 1997, 'Typing of Human Adenoviruses by Restriction Endonuclease Analysis and Enteric AdenovirusInfection Rate in Children with Gastroenteritis', Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisihttp://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=1997-2-3-195-207.pdf. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)