Mid2 depletion led to astrin stabilization during cytokinesis, cytokinetic defects, multinucleated cells, and cell death.
Ankur A. Gholkar, Silvia Senese, Yu-Chen Lo, Edmundo Vides, Ely Contreras, EmmanuelleHodara, Joseph Capri, Julian P. Whitelegge, Jorge Z. Torres 2016, 'The X-Linked-Intellectual-Disability-Associated Ubiquitin Ligase Mid2 Interacts withAstrin and Regulates Astrin Levels to Promote Cell Division', Cell Reportshttp://www.sciencedirect.com/science/article/pii/S221112471501459X. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
This cytokinetic defect might relate to its ability to bind microtubules, which was shown using an in vitro cosedimentation assay.
Audrey M. V. Ah-Fong, Howard S. Judelson 2011, 'New Role for Cdc14 Phosphatase: Localization to Basal Bodies in the Oomycete Phytophthoraand Its Evolutionary Coinheritance with Eukaryotic Flagella', PLoS ONE10.1371/journal.pone.0016725. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
This mechanism of cell division may resemble an ancestral mode of division in the absence of cytokinetic machinery.
Ignacio Flor-Parra, Manuel Bernal, Jacob Zhurinsky, Rafael R. Daga 2013, 'Cell migration and division in amoeboid-like fission yeast', Biology Openhttp://bio.biologists.org/content/3/1/108. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
These results indicate that this method could be a very useful tool for cytokinetic and pharmacological research.
Lin Qiu, Ming Liu, Konglun Pan 2013, 'A Triple Staining Method for Accurate Cell Cycle Analysis Using Multiparameter FlowCytometry', Moleculeshttp://www.mdpi.com/1420-3049/18/12/15412. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)