an agent which prevents cell damage caused by cryopreservation
If a cell is immersed in a solution of cryoprotectant, before the molecules can diffuseinto the cell osmotic forces dictate that water passes out of the cell
Examples of 'cryoprotectant' in a sentence
cryoprotectant
At 50%, the cryoprotectant has no longer a beneficial effect in the vitrification process.
Andrea Hettig, Vasile Miclea, Marius Zăhan, Iulian Roman, Ileana Miclea, Miklós Botha,Alexandru Rusu, Florin Varo-Ghiuru 2011, 'Vitrification of Immature Swine Oocytes by the SSV Technique using Different Concentrationof Cryoprotector and Exposure Time', Scientific Papers Animal Science and Biotechnologieshttp://spasb.ro/index.php/spasb/article/view/523. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
The effect depends on the cryoprotectant dose as well as the breed of bull.
Fabián L Rueda, Rocío F Herrera, Luis F Arbeláez, Tatiana Garcés, Henry Velasquez,Miguel A Peña, Jaime A Cardozo 2013, 'Increase in post-thaw viability by adding seminal plasma proteins to Sanmartineroand Zebu sperm', Revista Colombiana de Ciencias Pecuariashttp://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-06902013000200005&lng=en&tlng=en. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
No significant differences were observed among the cryoprotectant solutions.
Luis David Solis Murgas, Aléssio Batista Miliorini, Rilke Tadeu Fonseca de Freitas,Gilmara Junqueira Machado Pereira 2007, 'Criopreservação do sêmen de curimba (Prochilodus lineatus) mediante adição de diferentesdiluidores, ativadores e crioprotetores Cryopreservation of curimba (Prochilodus lineatus)semen after addition of different diluters, activators and cryoprotectants', Revista Brasileira de Zootecniahttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982007000300002. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
Viability over 24 hours was lower in concentrations of cryoprotectant higher than 10%.
Bianca Lawson, Simon Clulow, Michael J Mahony, John Clulow 2013, 'Towards gene banking amphibian maternal germ lines: short-term incubation, cryoprotectanttolerance and cryopreservation of embryonic cells of the frog, Limnodynastes peronii.',PLoS ONEhttp://europepmc.org/articles/PMC3618038?pdf=render. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
However, the cryoprotectant agent sucrose (68%) produces a preservative effect in cryopreserved rabies virus samples.
Tereza D'avila de Freitas Aguiar, Maria Fatima da Silva Teixeira, Edmara Chaves Costa,Allan Bezerra Vitaliano, Carlos Henrique de Andrade Teles, Igor Ciriaco Barroso, RonaldoPereira Dias, Nelio Batista de Moraes 2013, 'Medium-term cryopreservation of rabies virus samples', Revista da Sociedade Brasileira de Medicina Tropicalhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000600678&lng=en&tlng=en. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
In conclusion, in the experimental conditions used, glycerol showed more cryoprotectant potential than methyl-formamide.
Carlos Pereira das Graças, Alexandre In Piao Gomes Lim, Andrei Antonioni Guedes Fidelis,Júlio Roquete Cardoso, Hélio Blume, Rafael Gianella Mondadori 2013, 'METIL-FORMAMIDA NA CRIOPRESERVAÇÃO DE SÊMEN OVINO', Ciência Animal Brasileirahttp://www.revistas.ufg.br/index.php/vet/article/view/17835/15640. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
We also observe strong water isotope effects on ice crystallisation processes in the cryoprotectant mixture.
Oleg Kirichek, Alan Soper, Boris Dzyuba, Sam Callear, Barry Fuller 2015, 'Strong isotope effects on melting dynamics and ice crystallisation processes in cryovitrification solutions.', PLoS ONEhttp://europepmc.org/articles/PMC4376522?pdf=render. Retrieved from PLOS CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)
A preferred formula was lyophilized using mannitol as the cryoprotectant.
Mohaddeseh Dashti nejad pour, Mohammad Reza Khoshayand 2018, 'Fingolimod SLNs: Preparation, in vitro evaluation and Optimization of lyophilizationusing D-Optimal Experimental Design', Journal of Pharmaceutical and Health Scienceshttp://www.jphs.ir/article_543561.html. Retrieved from DOAJ CC BY 4.0 (https://creativecommons.org/licenses/by-sa/4.0/legalcode)