| 单词 | southern blot |
| 释义 | > as lemmasSouthern blot Used attributively (esp. in Southern blot, see blot n.1 Additions, Southern blotting, see blotting n. Additions) with reference to a technique for the identification of specific nucleotide sequences in DNA, in which fragments separated on an agarose gel are transferred directly to another medium on which assay is carried out. Cf. northern adj. 8.The second medium is usually a nitrocellulose membrane, and assay is performed by hybridization to a labelled DNA fragment comprising the complementary sequence. ΘΚΠ the world > life > biology > biological processes > immunogenesis > [noun] > immunological assays > blot procedures Southern blotting1977 Western blot1981 Western blotting1981 western1985 the world > life > biology > biological processes > immunogenesis > [adjective] > of immunological assays > of blot procedures Southern blot1977 1977 Cell 12 741/2 Screening experiments, designed to explore the repertoire of viral transcripts present in the cytoplasm of Ad2-infected cells, have been conducted with a novel variation of the Southern blotting technique. 1981 Analyt. Biochem. 116 237/1 In the last 5 years, the Southern blot technique..has become one of the most common techniques in molecular biology. 1990 Jrnl. Exper. Bot. 41 1047/2 The presence of the NPTII gene in this tissue was confirmed by Southern hybridization of the product. 1996 New Scientist 11 May 42/3 A ‘modified’ version of gel electrophoresis—Southern blotting—allowed these chunks to be identified and their lengths compared. 2000 L. K. Kreppel & G. W. Hart in M. Fukuda & O. Hindsgaul Molecular & Cellular Glycobiol. vi. 203 Southern blot analysis indicates that the rat..and human..enzymes are not members of a closely related multigene family. < as lemmas |
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