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immunoblotting
immunoblotting n. 
/ɪˌmjuːnə(ʊ)ˈblɒtɪŋ/, /ˈɪmjᵿnə(ʊ)ˌblɒtɪŋ/, /ɪˈmjuːnə(ʊ)ˌblɒtɪŋ/, /ᵻˈmjunoʊˌblɑdɪŋ/ a method for analysing proteins which involves their separation by electrophoresis followed by transfer to a membrane and reaction with (labelled) antibodies; = Western blotting at western adj. 8f.
Brit. /ˌɪmjᵿnə(ʊ)ˈblɒtɪŋ/
, /ˌɪmjᵿnə(ʊ)ˈblɒtɪŋ//ɪˌmjuːnə(ʊ)ˈblɒtɪŋ//ˈɪmjᵿnə(ʊ)ˌblɒtɪŋ//ɪˈmjuːnə(ʊ)ˌblɒtɪŋ/U.S. /ˈɪmjənoʊˌblɑdɪŋ/
, /ˈɪmjənoʊˌblɑdɪŋ//ᵻˈmjunoʊˌblɑdɪŋ/ΚΠ
1980 Jrnl. Cell Biol. 87 346 We searched for evidence of neurofilament proteins among the PSD polypeptides. This was done with a novel technique for detecting protein antigens in SDS-polyacrylamide gels (immunoblotting) and an antiserum that was selective for neurofilaments in immunohistochemical tests.
1990 EMBO Jrnl. 9 4005/1 Proteins for immunoblotting were separated in an SDS-18% polyacrylamide gel and transferred to nitrocellulose.
2005 Methods Molecular Biol. 295 227 Immunoblotting is now widely used in conjunction with two-dimensional polyacrylamide gel electrophoresis, not only for traditional goals..but also as a genome-proteome interface technique.
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