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单词 counterstain
释义 ˈcounterstain, n.
[counter- 3.]
An additional dye of different colour or sensitivity used in a microscopy specimen to produce a contrasting background to the parts of interest or to make clearer the distinction between different kinds of structure, tissue, etc. Hence as v. trans. (freq. absol.), to treat with a counterstain; to stain (a tissue or specimen) with a contrasting colour; ˈcounterˌstaining vbl. n.
1895Kanthack & Drysdale Elem. Pract. Bacteriol. 28 Stain a film according to Gram's method... Counterstain with eosine.1899G. Newman Bacteria 341 A pure culture of bacteria will not necessarily require the counter-stain (methylene-blue).1901Jrnl. Exper. Med. V. 559 Rehm counter-staining with a solution of carmine..stained the centre of the nucleolus red, while leaving a peripheral rim of blue.1951Whitby & Hynes Med. Bacteriol. (ed. 5) v. 34 Gram-negative organisms..have to be shown up by a contrast counterstain.Ibid. 35 Counterstain films from cultures with weak carbol-fuchsin.Ibid., After counter⁓staining dehydrate quickly in methylated spirit and then alcohol.1970Disbrey & Rack Histol. Lab. Methods vii. 78 The counterstaining solution should not itself remove any of the specific stain.Ibid. 79 Occasionally..the counterstain is used before the specific stain.
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