| 释义 |
Michaelis, n. Biochem.|mɪˈkeɪlɪs|
[The name of Leonor Michaelis (1875–1949), German-born American chemist.]
Michaelis constant, the concentration of a given enzyme which catalyses the associated reaction at half the maximum rate.
1930J. B. S. Haldane Enzymes iii. 39 We thus have two constants available for fitting a series of results, but V should be (and is found to be) proportional to the enzyme concentration, while Km, which is generally called the Michaelis constant, is a characteristic of the enzyme. 1955New Biol. XVIII. 63 The concentration of substrate that just gives the maximum rate [of reaction] is difficult to determine exactly, but the concentration giving the half-maximum rate, which is known as the Michaelis constant, can be determined with precision and is important in the characterization of enzymes. 1968New England Jrnl. Med. CCLXXVIII. 475/1 Starch substrate concentrations of 1.00, 0.70, 0.40, 0.25 gm per 100 ml were used to determine the Michaelis constants. 1987C. G. Sinclair in Bu'lock & Kristiansen Basic Biotechnol. iv. 91 Eo is the total amount of enzyme, km is the Michaelis constant, and S is the substrate concentration. |